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fitc anti mouse f4 80  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology fitc anti mouse f4 80
    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis <t>of</t> <t>F4/80</t> and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.
    Fitc Anti Mouse F4 80, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+f4+80/FITC+Anti-Mouse+F4%2F80+Antibody/pmc13091351-201-5-35
    Average 95 stars, based on 76 article reviews
    fitc anti mouse f4 80 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment"

    Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

    Journal: Molecular Therapy Oncology

    doi: 10.1016/j.omton.2026.201185

    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.
    Figure Legend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

    Techniques Used: Flow Cytometry

    Related Articles

    Staining:

    Article Title: IgG promotes TNF‐α induced osteoclastogenesis by upregulating the expression of TNFR1 and the NF‐κB signalling pathway
    Article Snippet: The secondary antibodies include HRP‐conjugated Affinipure Goat Anti‐Mouse IgG (dilution 1:5000, SA00001‐1, Proteintech, Wuhan, China) and HRP‐conjugated Affinipure Goat Anti‐Rabbit (dilution 1:5000, SA00001‐2, Proteintech, Wuhan, China). β‐actin was used as the control for total protein. .. Fluorochrome‐conjugated antibodies (5 uL per test) were used to stain BMDMs for 30 min, including FITC‐conjugated anti‐F4/80 (E‐AB‐F0995C, Elabscience, Wuhan, China) and PE‐conjugated TNF Receptor 1 (Biolegend, California, USA). .. The stained cells were measured by a FACScan flow cytometer (BD, New Jersey, USA).

    Article Title: Helminth reshapes host gut microbiota and immunoregulation by deploying an antimicrobial program of innate immunity
    Article Snippet: .. In brief, the cell pellets were incubated with Fixable Viability Dye (Cat. 65-0866-14, ThermoFisher Scientific, 100 μL/mL) for dead cells, and anti-CD16/32 mAbs (Cat. E-AB-F0997A, Elabscience, 50 μL/mL) to block Fc receptors, followed by staining with fluorescent antibodies against cell surface molecules for 1 h at 4°C, including antibodies of anti-F4/80 (Cat. E-AB-F0995C, Elabscience, 50 μL/mL), anti-CD11b (Cat. E-AB-F1081J, Elabscience, 50 μL/mL), and anti-CD86 (Cat. E-AB-F0994E, Elabscience, 50 μL/mL). .. For intracellular marker staining, cells were fixed and permeabilized using a Fixation/Permeabilization kit (Cat. 00-5223-56, Invitrogen, 100 μL/mL), and stained for anti-CD206 antibody (Cat. E-AB-F1135D, Elabscience, 100 μL/mL) at 4°C for 1 h. The prepared cells were analyzed by a flow cytometry machine (Beckman Coulter CytoFLEX-LX) to determine the cell populations of macrophages.

    Incubation:

    Article Title: Helminth reshapes host gut microbiota and immunoregulation by deploying an antimicrobial program of innate immunity
    Article Snippet: .. In brief, the cell pellets were incubated with Fixable Viability Dye (Cat. 65-0866-14, ThermoFisher Scientific, 100 μL/mL) for dead cells, and anti-CD16/32 mAbs (Cat. E-AB-F0997A, Elabscience, 50 μL/mL) to block Fc receptors, followed by staining with fluorescent antibodies against cell surface molecules for 1 h at 4°C, including antibodies of anti-F4/80 (Cat. E-AB-F0995C, Elabscience, 50 μL/mL), anti-CD11b (Cat. E-AB-F1081J, Elabscience, 50 μL/mL), and anti-CD86 (Cat. E-AB-F0994E, Elabscience, 50 μL/mL). .. For intracellular marker staining, cells were fixed and permeabilized using a Fixation/Permeabilization kit (Cat. 00-5223-56, Invitrogen, 100 μL/mL), and stained for anti-CD206 antibody (Cat. E-AB-F1135D, Elabscience, 100 μL/mL) at 4°C for 1 h. The prepared cells were analyzed by a flow cytometry machine (Beckman Coulter CytoFLEX-LX) to determine the cell populations of macrophages.

    Blocking Assay:

    Article Title: Helminth reshapes host gut microbiota and immunoregulation by deploying an antimicrobial program of innate immunity
    Article Snippet: .. In brief, the cell pellets were incubated with Fixable Viability Dye (Cat. 65-0866-14, ThermoFisher Scientific, 100 μL/mL) for dead cells, and anti-CD16/32 mAbs (Cat. E-AB-F0997A, Elabscience, 50 μL/mL) to block Fc receptors, followed by staining with fluorescent antibodies against cell surface molecules for 1 h at 4°C, including antibodies of anti-F4/80 (Cat. E-AB-F0995C, Elabscience, 50 μL/mL), anti-CD11b (Cat. E-AB-F1081J, Elabscience, 50 μL/mL), and anti-CD86 (Cat. E-AB-F0994E, Elabscience, 50 μL/mL). .. For intracellular marker staining, cells were fixed and permeabilized using a Fixation/Permeabilization kit (Cat. 00-5223-56, Invitrogen, 100 μL/mL), and stained for anti-CD206 antibody (Cat. E-AB-F1135D, Elabscience, 100 μL/mL) at 4°C for 1 h. The prepared cells were analyzed by a flow cytometry machine (Beckman Coulter CytoFLEX-LX) to determine the cell populations of macrophages.

    other:




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    Elabscience Biotechnology fitc anti mouse f4 80
    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis <t>of</t> <t>F4/80</t> and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.
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    Image Search Results


    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

    Journal: Molecular Therapy Oncology

    Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

    doi: 10.1016/j.omton.2026.201185

    Figure Lengend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

    Article Snippet: The following antibodies were used: FITC anti-mouse F4/80 (clone CI: A3-1), APC anti-mouse CD86 (clone GL-1), FITC anti-mouse CD3 (clone 17A2), APC anti-mouse CD4 (clone GK1.5), and APC anti-mouse CD8 (clone YTS-169), all purchased from Elabscience Biotechnology Co., Ltd.

    Techniques: Flow Cytometry